L-012化學發光探針
生化學用 for Biochemistry3116094040028274.png 規格含量 : 90.0+%（HPLC） 制造商 : FUJIFILM Wako Pure Chemical Corporation（富士膠片和光） 儲存條件：冷藏（干冰運輸） CAS RN? : 143556-24-5 分子式 : C13H8ClN4NaO2 分子量 : 310.67 |  |
◆基本信息
L-012 是一種高靈敏度的化學發光（CHL）探針，比魯米諾（Luminol）試劑更有效。L-012 與多種類型的活性氧反應，這些活性氧來自人血液和口腔中激活的中性粒細胞或者大鼠腹膜腔。該產品可以用于有辣根過氧化物酶的ELISA 試劑盒，可提高反應靈敏度。
結構式
溶解及保存：蒸餾水溶解成20 mM/L（6.23 mg/mL）的水溶液，再用50 mM/L的Tris鹽酸緩沖液（pH 7.5）稀釋 至最終濃度為500 μM/L（有細胞的反應體系）。另外，在沒細胞參與的反應體系中時，建議使用終濃度為5~50 μM/L。母液冷凍保存，使用時再稀釋。
◆物性信息
外觀 | 白色~黃色，結晶~粉末 |
溶解性 | 可溶于水。 |
| 產品編號 | 產品名稱 | 產品規格 | 產品等級 |
| 120-04891 | L-012 L-012化學發光探針 | 100 mg | － |
拜爾迪是FUJIFILM wako授權代理商，
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Q&A
Q： L-012能滲入細胞嗎?
A： 由于L-012對脂質高度親和，它比luminol和lucigenin更能具有細胞滲透性。另一方面，ROS無限制地在胞外釋放。因此L-012被認為也能
檢測胞外ROS。
Q： L-012的使用濃度如何?
A： 這取決于應用和條件。
[體外] 培養細胞（EoL-1細胞）： 500 ~ 800 μmol/L.
全血和分離的中性粒細胞： 400 μmol/L
多形核中性粒細胞（PMNs）（由小鼠中分離）： 2 mmol/L
無細胞的樣品： 4 μmol/L, 15 μmol/L, and 50 μmol/L
[體內] Balb/c 小鼠[皮下]： 20 ~ 80 μL of L-012(5mg/mL)
Balb/c 小鼠[腹膜內]： 100 μL of L-012 (15mg/mL)
C57BL/10.Q (B10.Q) 小鼠 [腹膜內]： 1 ~ 75 mg/kg
Q： 是否可用于動物活體成像？
A： 如果所用的小動物成像儀中有發光檢測探頭，就可以進行檢測。以下是文獻報道過的儀器型號：
?IVIS 100 imaging systems
?Infinite M200 [Tecan]
?Luminescence Reader BLR-201 [Aloka]
?Beckman-Coulter DTX880 multimode plate reader [Beckman]
Q： 是否可用于固定細胞？
A： 目前尚無證據表明，活性氧（ROS）在細胞活性停止后仍然存在。普遍看法是認為在細胞活性停止后（細胞死亡）ROS消失。固定組織或
細胞會停止細胞活性，因此不建議用于ROS檢測。
本品無需固定檢測對象即可檢測到ROS，使用方法包括但不限于將其添加到細胞培養上清液中或進行動物腹腔注射。
參考文獻
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