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BN40863R-100ul
100ul
￥2470.00
交叉反應(yīng)：Human(predicted:Mouse,Rat,Chicken,Dog,Pig,Cow,Horse,Rabbit) 推薦應(yīng)用：WB,Flow-Cyt,ELISA
產(chǎn)品描述
| 英文名稱 | phospho-Cyclin E1 (Thr77) |
| 中文名稱 | 磷酸化周期素E1抗體 |
| 別 名 | Cyclin E1 (phospho-Thr77); Cyclin E1 (phospho-T77); CCNE 1; CCNE; CCNE1; Cyclin Es; Cyclin Et; CyclinE; G1/S specific cyclin E; G1/S-specific cyclin-E1; CCNE1_HUMAN; pCCNE1. |
| 產(chǎn)品類型 | 磷酸化抗體 |
| 研究領(lǐng)域 | 腫瘤 細(xì)胞生物 細(xì)胞周期蛋白 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human, (predicted: Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, ) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000 ELISA=1:5000-10000 Flow-Cyt=1μg /Test not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 47kDa |
| 細(xì)胞定位 | 細(xì)胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated Synthesised phosphopeptide derived from human Cyclin E around the phosphorylation site of Thr77:IP(p-T)PD |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲(chǔ) 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 產(chǎn)品介紹 | Cyclin E is a regulatory subunit of Cdk2 and controls G1 / S transition during the mammalian cell cycle. Multiple isoforms of Cyclin E are only expressed in tumors but not in normal tissue, suggesting a post transcriptional regulation of Cyclin E. In vitro analyses indicated that these truncated variant isoforms of Cyclin E are able to phosphorylate histone H1. Alterations in the Cyclin E protein have been implicated as indicators of worse prognosis in various cancers. Function: Essential for the control of the cell cycle at the G1/S (start) transition. Subunit: Interacts with a member of the CDK2/CDK protein kinases to form a serine/threonine kinase holoenzyme complex. The cyclin subunit imparts substrate specificity to the complex. Found in a complex with CDK2, CABLES1 and CCNA1 (By similarity). Part of a complex consisting of UHRF2, CDK2 and CCNE1. Interacts directly with UHRF2; the interaction ubiquitinates CCNE1 and appears to occur independently of CCNE1 phosphorylation. Subcellular Location: Nucleus. Tissue Specificity: Highly expressed in testis and placenta. Low levels in bronchial epithelial cells. Post-translational modifications: Phosphorylation of Thr-395 by GSK3 and of Ser-399 by CDK2 accelerates degradation via the ubiquitin proteasome pathway. Phosphorylated upon DNA damage, probably by ATM or ATR. Ubiquitinated by UHRF2; appears to occur independently of phosphorylation. Similarity: Belongs to the cyclin family. Cyclin E subfamily. SWISS: P24864 Gene ID: 898 Database links: Entrez Gene: 898 Human Entrez Gene: 12447 Mouse Omim: 123837 Human SwissProt: P24864 Human SwissProt: Q61457 Mouse Unigene: 244723 Human Unigene: 16110 Mouse Unigene: 15455 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 細(xì)胞周期素E是調(diào)控細(xì)胞G-1→S期轉(zhuǎn)變的關(guān)鍵因素。由于在多種腫瘤中的不適當(dāng)表達(dá),細(xì)胞周期素E現(xiàn)在已明確為原癌基因。 |
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